Daramy, K; Punnabhum, P; Hussain, M; Minelli, C; Pei, Y; Rattray, N J W; Perrie, Y; Rattray, Z (2024) Nanoparticle Isolation from Biological Media for Protein Corona Analysis: The Impact of Incubation and Recovery Protocols on Nanoparticle Properties. Journal of Pharmaceutical Sciences, 113 (9). pp. 2826-2836. ISSN 00223549

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Abstract

Nanoparticles are increasingly implemented in biomedical applications, including the diagnosis and treatment of disease. When exposed to complex biological media, nanoparticles spontaneously interact with their surrounding environment, leading to the surface-adsorption of small and bio- macromolecules- termed the “corona”. Corona composition is governed by nanoparticle properties and incubation parameters. While the focus of most studies is on the protein signature of the nanoparticle corona, the impact of experimental protocols on nanoparticle size in the presence of complex biological media, and the impact of nanoparticle recovery from biological media has not yet been reported. Here using a non-degradable robust model, we show how centrifugation-resuspension protocols used for the isolation of nanoparticles from incubation media, incubation duration and shear flow conditions alter nanoparticle parameters including particle size, zeta potential and total protein content.

Item Type:	Article
Keywords:	nanoparticle, protein corona, flo conditions, Polymeric, Particle size, Agglomeration
Subjects:	Nanoscience > Surface and Nanoanalysis
Divisions:	Chemical & Biological Sciences
Identification number/DOI:	10.1016/j.xphs.2023.12.021
Last Modified:	25 Mar 2026 15:53
URI:	https://eprintspublications.npl.co.uk/id/eprint/10363