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Exploring new methods to study and moderate proton beam damage for multimodal imaging on a single tissue section

Costa, C; de Jesus, J; Nikula, C; Murta, T; Grime, G W; Palitsin, V; Webb, R; Goodwin, R J A; Bunch, J (2022) Exploring new methods to study and moderate proton beam damage for multimodal imaging on a single tissue section. Journal of the American Society for Mass Spectrometry, 33 (12). pp. 2263-2272.

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Abstract

Characterising proton beam damage in biological materials is of interest to enable the integration of proton microprobe elemental mapping techniques with other imaging modalities. It is also of relevance to obtain a deeper understanding of changes that take place to tissues during proton beam cancer therapy. We have developed a novel strategy to characterise proton beam damage to lipids in biological tissues, based on mass spectrometry imaging. This methodology is applied to characterise changes to lipids in tissues irradiated under different conditions designed to mitigate beam damage. This work shows that performing proton beam irradiation at ambient pressure, as well as including an application of an organic matrix prior to irradiation can reduce damage to lipids in tissues. We also discovered that, irrespective of proton beam irradiation, placing a sample in vacuum prior to desorption electrospray ionisation (DESI) imaging can enhance lipid signals, a conclusion that may be of future benefit to the mass spectrometry imaging (MSI) community.

Item Type: Article
Subjects: Nanoscience > Surface and Nanoanalysis
Divisions: Chemical & Biological Sciences
Identification number/DOI: 10.1021/jasms.2c00226
Last Modified: 08 Feb 2023 15:13
URI: http://eprintspublications.npl.co.uk/id/eprint/9642

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