Prakash, K (2022) At the molecular resolution with MINFLUX? Philosophical Transactions of the Royal Society A Mathematical Physical and Engineering Sciences, 380 (2220). 20200145

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Abstract

MINFLUX is purported as the next revolutionary fluorescence microscopy technique claiming a spatial resolution in the range of 1–3nm in fixed and living cells. Though the claim of molecular resolution is attractive, I am concerned whether true 1nm resolution has been attained. Here, I compare the performance with other super-resolution methods focusing particularly on spatial resolution claims, subjective filtering of localizations, detection versus labelling efficiency and the possible limitations when imaging biological samples containing densely labelled structures. I hope the analysis and evaluation parameters presented here are not only useful for future research directions for single-molecule techniques but also microscope users, developers and core facility managers when deciding on an investment for the next ‘state-of-the-art’ instrument This article is part of the Theo Murphy meeting issue ‘Super-resolution structured illumination microscopy (part 2)’.

Item Type:	Article
Keywords:	MINFLUX, STED, SMLM, localization precision, image resolution, super-resolution imaging
Subjects:	Biotechnology > Biopharmaceutical Manufacturing and Characterisation
Divisions:	Chemical & Biological Sciences
Identification number/DOI:	10.1098/rsta.2020.0145
Last Modified:	15 Nov 2022 14:42
URI:	https://eprintspublications.npl.co.uk/id/eprint/9598