Belsey, N A; Shard, A G; Minelli, C (2015) Analysis of protein coatings on gold nanoparticles by XPS and liquid-based particle sizing techniques. Biointerphases, 10 (1). 019012

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Abstract

The precise use of nanoparticles in different technological applications requires control over their surface properties. This implies the ability to quantitatively describe NP molecular coatings in terms of their thickness, areal mass and number of molecules. Here, we describe two different approaches to the measurement of these parameters by using gold NPs ranging in size from 10 nm to 80 nm and coated with three different proteins: Immunoglobulin G (IgG); bovine serum albumin (BSA) and; a peptide. One approach utilises UV-Vis spectroscopy and the liquid-based techniques of dynamic light scattering (DLS) and differential centrifugal sedimentation (DCS) to measure the protein shell refractive indices, from which the number of molecules in the protein shell can be derived. The other approach utilised X-ray photoelectron spectroscopy (XPS) to measure the thickness of the NP shells and also to derive the number of molecules in the protein shell. We demonstrate that the two approaches, although very different, produce consistent measurement results. This finding is important to extend the quantitative analysis of nanoparticle molecular coatings to a wide range of NP materials.

Item Type:	Article
Subjects:	Nanoscience Nanoscience > Surface and Nanoanalysis
Identification number/DOI:	10.1116/1.4913566
Last Modified:	02 Feb 2018 13:13
URI:	http://eprintspublications.npl.co.uk/id/eprint/6551

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