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Abstract

When nanoparticles are introduced into biological media, they acquire a protein corona whose characterization is key to the full exploitation of their application. Here, we compare the use of dynamic light scattering (DLS), differential centrifugal sedimentation (DCS) and small angle X-ray scattering (SAXS) for the characterization of 105 nm polystyrene particles coated with proteins. All techniques measured an increase in protein shell thickness with increasing concentration of the proteins during incubation with the nanoparticles. DLS measurements were the highest in value, indicating a possible effect of particle agglomeration on the accuracy of the technique. DCS accuracy critically depended on the knowledge of the protein shell density, but allowed estimation of the effective density of the polymer core and protein shell. SAXS measurements proved powerful for understanding the complex core/shell structure of the particles, but model refinement of SAXS and DCS is required for the full consistency of the techniques

Item Type:	Article
Keywords:	nanoparticle, size measurement, protein coating, core/shell, DLS, DCS, SAXS, DTT
Subjects:	Nanoscience Nanoscience > Surface and Nanoanalysis
Identification number/DOI:	10.1002/sia.5381
Last Modified:	02 Feb 2018 13:14
URI:	http://eprintspublications.npl.co.uk/id/eprint/6460

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