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Differentiation between isomeric triantennary N-linked glycans by negative ion tandem mass spectrometry and confirmation of glycans containing galactose attached to the bisecting ß(1-4-GlcNAc) residue in N-glycans from IgG.

Harvey, D J*; Crispin, M*; Scanlan, C*; Singer, B B*; Lucka, L*; Chang, V T*; Radcliffe, C M*; Thobhani, S; Yuen, C-T*; Rudd, P M* (2008) Differentiation between isomeric triantennary N-linked glycans by negative ion tandem mass spectrometry and confirmation of glycans containing galactose attached to the bisecting ß(1-4-GlcNAc) residue in N-glycans from IgG. Rapid Commun. Mass Spectrom., 22 (7). pp. 1047-1052.

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Abstract

Negative ion tandem mass spectrometry (MS/MS) spectra of three isomeric triantennary N-linked glycans provided clear differentiation between the isomers and confirmed the occurrence of an isomer that was substituted with galactose on a bisecting GlcNAc (1 4-substituted on the core mannose) residue recently reported by Takegawa et al. from N-glycans released from human immunoglobulin G (IgG). We extend this analysis of human serum IgG to reveal an analogue of the fucosylated triantennary glycan reported by Takegawa et al. together with a third compound that lacked both the sialic acid and the fucose residues. In addition, we demonstrate the biosynthesis of bisected hybrid-type glycans with the galactose modification, with and without core fucose, on the stem cell marker glycoprotein, 19A, expressed in a partially ricin-resistant human embryonic kidney cell line. It would appear, therefore, that this modification of N-linked glycans containing a galactosylated bisecting GlcNAc residue may be more common than originally thought. Negative ion MS/MS analysis of glycans is likely to prove an invaluable tool in the analysis and monitoring of therapeutic glycoproteins.

Item Type: Article
Keywords: Glycans, mass spectrometry
Subjects: Biotechnology
Biotechnology > Biopharmaceutical Manufacturing and Characterisation
Last Modified: 02 Feb 2018 13:15
URI: http://eprintspublications.npl.co.uk/id/eprint/4551

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