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Protein quantitation assays for biopharmaceutical applications.

Noble, J E; Knight, A E; Reason, A J*; Di Matola, A*; Bailey, M J A (2007) Protein quantitation assays for biopharmaceutical applications. NPL Report. AS 11

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This report presents a review of current methods for estimation of protein amount in biological solutions, and a comparison of dye-based protein assays for biopharmaceutical analysis. The review details the various methods to determine the amount of protein in an aqueous sample, and highlights the limitations of some of these methods. The comparison of dye-based protein quantitation assays looks at various aspects of these spectroscopic methods in relation to the analysis of biopharmaceuticals including; accuracy, repeatability, dynamic range etc. The report contains recommendations for the use of specific dye-based assays for certain applications.

Item Type: Report/Guide (NPL Report)
NPL Report No.: AS 11
Subjects: Biotechnology
Biotechnology > Biopharmaceutical Manufacturing and Characterisation
Last Modified: 02 Feb 2018 13:15
URI: http://eprintspublications.npl.co.uk/id/eprint/3958

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