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Techniques for the measurement of the structure of proteins in solution, and their complementarity to circular dichroism.

Ravi, J; Tantra, R; Knight, A (2005) Techniques for the measurement of the structure of proteins in solution, and their complementarity to circular dichroism. NPL Report. DQL-AS 023

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Abstract

There is a recognised requirement for techniques complementary to Circular Dichroism (CD) for the structural characterisation of biopharmaceuticals as part of the quality control process. This report reviews the available techniques for protein structural characterisation and evaluates their suitability for this application. It is concluded that Fourier Transform Infrared Spectroscopy (FTIR) is the most suitable such technique.

Item Type: Report/Guide (NPL Report)
NPL Report No.: DQL-AS 023
Subjects: Biotechnology
Biotechnology > Biopharmaceutical Manufacturing and Characterisation
Last Modified: 02 Feb 2018 13:16
URI: http://eprintspublications.npl.co.uk/id/eprint/3436

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