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Electrchemical study of biotin-modified self-assembled monolayers.

Brett, D J L*; Brown, R J C (2005) Electrchemical study of biotin-modified self-assembled monolayers. NPL Report. DQL-AS 013

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Abstract

This report describes work carried out in support of the research project, ‘Controlled immobilisation of bimolecules on surfaces’. This work has developed the underpinning methodology for the production of robust, well-formed and densely packed, biotin-HPDP functionalised gold surfaces – the crucial first step in immobilising bimolecules on surfaces.
Self-Assembled Monolayers (SAMs) with biotin end-groups were prepared on polycrystalline gold surfaces according to a published method. The layers formed were studied using cyclic voltammetry to determine the composition of the layer and its quality. Crystal impedance spectroscopy was also applied as a complimentary indicator of the composition of the layer.
The effect of assembly time on the properties of the layer was studied along with the composition of the layer and the ability of the precursor molecule to self-assemble by oxidative addition.

Item Type: Report/Guide (NPL Report)
NPL Report No.: DQL-AS 013
Keywords: surfaces
Subjects: Biotechnology
Last Modified: 02 Feb 2018 13:16
URI: http://eprintspublications.npl.co.uk/id/eprint/3113

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