Steven, R T; Burton, A; Taylor, A J; Robinson, K N; Dexter, A; Nikula, C; Bunch, J (2024) Evaluation of inlet temperature with three different sprayer designs for desorption electrospray ionization mass spectrometry imaging of tissue analysis. Journal of The American Society for Mass Spectrometry, 35 (2). pp. 224-233.

Full text not available from this repository.

Abstract

Mass spectrometry imaging (MSI) provides spatially resolved detection of endogenous and exogenous molecules and atoms in biological samples, typically prepared as thin tissue sections. Desorption electrospray ionisation (DESI) MSI one of most established modalities used in pre-clinical research. DESI ion source technology is still rapidly evolving with new sprayer designs and heated inlet capillaries having recently been incorporated in commercially available systems. Within this study three iterations of DESI sprayer are evaluated: 1) the first, and until recently only, Waters commercial sprayer; 2) a developmental desorption electroflow focussing (DEFFI) type sprayer; 3) a prototype of the newly released Waters commercial sprayer. A heated inlet capillary is also employed allowing controlled inlet temperatures up to 500 °C. These three sprayers are evaluated by comparative tissue imaging analyses of murine testes across this temperature range. Single ion intensity vs temperature trends are evaluated as exemplar cases for putatively identified species of interest such as lactate and glutamine. A range of trends are observed where intensities follow either increasing, decreasing, bell shaped or flat responses with temperature. Data for all sprayers show approximately similar trends for the ions studied with the version 3 commercial prototype sprayer matching or outperforming the other sprayers for ions investigated. Finally, the mass spectra acquired using sprayer version 3 are evaluated by uniform manifold approximation and projection (UMAP) and k-means clustering. This approach is shown to provide valuable insight, complementary to the approach of univariate evaluation for reviewing the parameter space from this study. Full spectral temperature optimisation data are provided as supporting data to enable other researchers to design experiments optimal for specific ions.

Item Type:	Article
Subjects:	Nanoscience > Surface and Nanoanalysis
Divisions:	Chemical & Biological Sciences
Identification number/DOI:	10.1021/jasms.3c00332
Last Modified:	09 Sep 2024 13:45
URI:	https://eprintspublications.npl.co.uk/id/eprint/10003