Al-Rekabi, Z; Dondi, C; Faruqui, N; Siddiqui, N S; Elowsson, L; Rissler, J; Karedal, M; Mudway, I; Larsson-Callerfelt, A-K; Shaw, M (2023) Uncovering the cytotoxic effects of air pollution with multimodal imaging of in vitro respiratory models. Royal Society Open Science, 10 (4). 221426

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Abstract

Annually, an estimated seven million deaths are linked to exposure to airborne pollutants. Despite extensive epidemiological evidence supporting clear associations between poor air quality and a range of short- and long-term health effects, there are considerable gaps in our understanding of the specific mechanisms by which pollutant exposure induces adverse biological responses at the cellular and tissue levels. The development of more complex, predictive, in vitro respiratory models, including 2D and 3D cell cultures, spheroids, organoids, and tissue cultures, along with more realistic aerosol exposure systems, offers new opportunities to investigate the cytotoxic effects of airborne particulates under controlled laboratory conditions. Parallel advances in high-resolution microscopy have resulted in a range of in vitro imaging tools capable of visualising and analysing biological systems across unprecedented scales of length, time and complexity. This article considers state-of-the-art in vitro respiratory models and aerosol exposure systems and how they can be interrogated using high-resolution microscopy techniques to investigate cell-pollutant interactions, from the uptake and trafficking of particles to structural and functional modification of subcellular organelles and cells. These data can provide a mechanistic basis from which to advance our understanding of the health effects of airborne particulate pollution and develop improved mitigation measures.

Item Type:	Article
Subjects:	Biotechnology > Bio-Diagnostics
Divisions:	Chemical & Biological Sciences
Identification number/DOI:	10.1098/rsos.221426
Last Modified:	08 Apr 2024 15:00
URI:	https://eprintspublications.npl.co.uk/id/eprint/9956