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Investigation of mechanosensation in C. elegans using light field calcium imaging.

Shaw, M J; Elmi, M*; Pawar, V*; Srinivasan, M A* (2016) Investigation of mechanosensation in C. elegans using light field calcium imaging. Biomed. Opt. Express, 7 (7). pp. 2877-2887.

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Abstract

We describe a new experimental approach to investigate touch sensation in the model organism C. elegans using light field deconvolution microscopy. By combining fast volumetric image acquisition with controlled indentation of the organism using a high sensitivity force transducer we are able to simultaneously measure activity in multiple touch receptor neurons expressing the calcium ion indicator GCaMP6s. By varying the applied mechanical stimulus we show how this method can be used to quantify touch sensitivity in C. elegans. We describe some of the challenges of performing light field calcium imaging in moving samples and demonstrate that they can be overcome by simple data processing.

Item Type: Article
Keywords: Fluorescence microscopy, Three-dimensional microscopy, Medical and biological imaging
Subjects: Biotechnology
Biotechnology > Bio-Diagnostics
Identification number/DOI: 10.1364/BOE.7.002877
Last Modified: 02 Feb 2018 13:13
URI: http://eprintspublications.npl.co.uk/id/eprint/7169

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