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Aptamer-mediated detection of Thrombin using silver nanoparticle signal enhancement.

Szymanski, M; Noble, J; Knight, A; Porter, R A; Worsley, G J (2013) Aptamer-mediated detection of Thrombin using silver nanoparticle signal enhancement. Anal. Methods, 19 (1). pp. 187-191.

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Abstract

We present the first assay combining a dual aptamer sandwich format with detection by anodic stripping voltammetry with ionic silver amplification. This assay format lends itself to rapid point-of-care tests, where the use of aptamers could improve the overall stability of the assay. We have used human alpha-thrombin as a model system, and demonstrate a detection limit of 7.88 µg/L. We present the optimization of the aptamer-silver colloid attachment chemistry and the final assay format to achieve sensitive analyte detection. The use of a sandwich assay coupled with magnetic separation and ionic silver amplification, generates an assay with similar sensitivity than those reported in the literature in a format that can be used in rapid, portable testing regimes.

Item Type: Article
Keywords: Aptamer, Thrombin, Anodic striping voltammetry, Electrochemical detection, Voltammetry
Subjects: Biotechnology
Biotechnology > Bio-Diagnostics
Identification number/DOI: 10.1039/c2ay25906k
Last Modified: 02 Feb 2018 13:14
URI: http://eprintspublications.npl.co.uk/id/eprint/5669

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