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Rapid duplex immunoassay for wound biomarkers at the point-of-care.

Worsley, G J; Attree, S L; Noble, J E; Horgan, A M (2012) Rapid duplex immunoassay for wound biomarkers at the point-of-care. Biosens. Bioelectron., 34 (1). pp. 215-220.

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Abstract

In this study we describe a novel method of sampling and quantifying wound biomarkers in clinical settings. We believe the chosen format will allow rapid assessments of wound healing and provide biomarker evidence-based decision points for treatment of the wound at the time of presentation. The wound monitoring principle uses a proprietary sample collection tool (a thermally reversible hydrogel) to sample and isolate biomarkers within a wound environment without further sample extraction/preparation steps. We show how gel samples can be analysed in a lateral flow assay format utilising fluorescent microspheres with optically discrete emission characteristics and demonstrate quantitative detection of two analytes (duplexing) achieved in a single test line. As a model assay, the chronic wound biomarkers Interleukin 6 and Tumour Necrosis Factor alpha are used and limits of detection of 7.15 pg/mL and 10.7 pg/mL reported. We believe this is the first literature example of quantitative detection of multiple analytes within a single test line using spectral separation to distinguish the analytes.

Item Type: Article
Subjects: Biotechnology
Biotechnology > Bio-Diagnostics
Identification number/DOI: 10.1016/j.bios.2012.02.005
Last Modified: 02 Feb 2018 13:14
URI: http://eprintspublications.npl.co.uk/id/eprint/5500

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