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Competitive adsorption of macromolecules and real time dynamics of the Vroman-like effect.

Gun'ko, V M*; Mikhalovska, L I*; Tomlins, P E; Mikhalvosky, S V* (2011) Competitive adsorption of macromolecules and real time dynamics of the Vroman-like effect. Phys. Chem. Chem. Phys., 13 (10). pp. 4476-4485.

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Abstract: Quasi-elastic light scattering (QELS) and kinetic (quartz crystal microbalance, QCM) non-equilibrium and equilibrium adsorption studies of competitive interactions of pairs of a polymer (PVP, PEG, PVA) and a protein (BSA, gelatin, ovalbumin) with fumed silica in aqueous solutions or a protein pair (Fg, HSA) with gold, titanium, diamond like carbon (DLC), and TiN thin films coated onto the QCM sensors show complex interfacial behavior of the systems studied. The observed effects depend on the adsorption order of co-adsorbates, their chemical structure, adsorbent morphology and observation time. QCM applied to monitor the dynamics of the Vroman effect on adsorption of plasma proteins to various surfaces shows that in contrast to albumin (HSA), fibrinogen (Fg) can replace a fraction of pre-adsorbed HSA depending on nano-relief and hydrophilic/hydrophobic properties of solid surfaces. The equilibrium adsorption and the dynamics of interactions of macromolecules with uncovered or polymer (or protein) covered surfaces were compared in terms of the distribution functions of Gibbs free energy of adsorption (DG) varied over a relatively broad range from DG = -25 kJ/mol (adsorption onto uncovered surface) to 0 kJ/mol (polymer/protein pre-covered surface).

Item Type: Article
Keywords: Protein adsorption, vroman effect, qcm
Subjects: Advanced Materials
Advanced Materials > Biomaterials
Identification number/DOI: 10.1039/c0cp02165b
Last Modified: 02 Feb 2018 13:14
URI: http://eprintspublications.npl.co.uk/id/eprint/4900

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