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Fluorescent labelling of protein analysis.

Noble, J E; Cerasoli, E (2007) Fluorescent labelling of protein analysis. NPL Report. AS 10

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Abstract

This report compares fluorescent labelling methods to tag an antibody of an important cardiac biomarker. We have defined the characteristics of an ideal fluorescent labelling tag as one that retains the biological activity, compared to the unlabelled protein and generates a bright fluorescent tag that can be detected at low concentrations. Depending on the end application that the fluorescently labelled protein is required for will determine the number of positional requirement of the label. The four labelling chemistries analysed included amine, thiol and oligosaccharide-directed and biotinylation, all with the Cy3 dye. These labelling chemistries have been assessed in term of binding kinetics, relative quantum yield and labelling ratio, with discussion based on suitability for specific applications.

Item Type: Report/Guide (NPL Report)
NPL Report No.: AS 10
Subjects: Biotechnology
Biotechnology > Biopharmaceutical Manufacturing and Characterisation
Last Modified: 02 Feb 2018 13:15
URI: http://eprintspublications.npl.co.uk/id/eprint/3957

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