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Microscope calibration using laser written fluorescence

Corbett, A D; Shaw, M; Yacoot, A; Jefferson, A; Schermelleh, L; Wilson, T; Booth, M; Salter, P S (2018) Microscope calibration using laser written fluorescence. Optics Express, 26 (17). pp. 21887-21899. ISSN 1094-4087

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Abstract

There is currently no widely adopted standard for the optical characterisation of fluorescence microscopes. We used laser written fluorescence to generate two- and three-dimensional patterns to deliver a quick and quantitative measure of imaging performance. We report on the use of two laser written patterns to measure the lateral resolution, illumination uniformity, lens distortion and colour plane alignment using confocal and structured illumination fluorescence microscopes.

Item Type: Article
Keywords: Microscopy, standards, fluorescence
Subjects: Engineering Measurements > Dimensional
Divisions: Chemical, Medical & Environmental Science
Identification number/DOI: 10.1364/OE.26.021887
Last Modified: 08 Oct 2018 13:16
URI: http://eprintspublications.npl.co.uk/id/eprint/8093

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