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DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle

Mikheikin, A; Olsen, A; Leslie, K; Russell-Pavier, F; Yacoot, A; Picco, L; Payton, O; Toor, A; Chesney, A; Gimzewski, J K; Mishra, B; Reed, J (2017) DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle. Nature Communications, 8. 1665

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Abstract

We present an enabling advance in single molecule genomics with the potential to place mapping and next-generation sequencing on the same accelerated technological growth curves. Key results include close to single base pair (¿bp¿) resolution in sizing PCR amplicons, ultra-fine mapping of short sequences on long-PCR molecules from the BRCA1 locus (e.g., 12,900 bp long), and a cost-scalable instrument using commercial DVD player parts.

Item Type: Article
Keywords: DNA, AFM high speed
Subjects: Engineering Measurements > Dimensional
Divisions: Engineering, Materials & Electrical Science
Identification number/DOI: 10.1038/s41467-017-01891-9
Last Modified: 08 Mar 2018 14:36
URI: http://eprintspublications.npl.co.uk/id/eprint/7793

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